Product Name
Ketohexokinase (KHK), Polyclonal Antibody
Full Product Name
Anti-Ketohexokinase Antibody
Product Synonym Names
Ketohexokinase; Hepatic fructokinase
Product Gene Name
anti-KHK antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P50053
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Ketohexokinase protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
Recombinant full length protein of human Ketohexokinase
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-KHK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KHK antibody
Rabbit polyclonal antibody to Ketohexokinase
Applications Tested/Suitable for anti-KHK antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-KHK antibody
WB: 1/500 - 1/2000; IHC: 1/50 - 1/200
Western Blot (WB) of anti-KHK antibody
Western blot analysis of Ketohexokinase expression in mouse liver (A), rat kidney (B) whole cell lysates.
Immunohistochemistry (IHC) of anti-KHK antibody
Immunohistochemical analysis of Ketohexokinase staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
NCBI/Uniprot data below describe general gene information for KHK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000212.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000221.2
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UniProt Primary Accession #
P50053
[Other Products]
UniProt Secondary Accession #
Q6IBK2; Q99532; Q9BRJ3; Q9UMN1[Other Products]
UniProt Related Accession #
P50053[Other Products]
Molecular Weight
32,730 Da
NCBI Official Full Name
ketohexokinase isoform a
NCBI Official Synonym Full Names
ketohexokinase
NCBI Official Symbol
KHK [Similar Products]
NCBI Protein Information
ketohexokinase
UniProt Protein Name
Ketohexokinase
UniProt Synonym Protein Names
Hepatic fructokinase
Protein Family
Ketohexokinase
UniProt Gene Name
KHK [Similar Products]
UniProt Entry Name
KHK_HUMAN
NCBI Summary for KHK
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for KHK
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
Research Articles on KHK
1. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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