Full Product Name
KHK antibody
Product Synonym Names
Monoclonal KHK; Anti-KHK; Ketohexokinase; Fructokinase
Product Gene Name
anti-KHK antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P50053
Purity/Purification
KHK antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (lot specific)
Biological Significance
KHK is a ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver, renal cortex, and small intestine, while the alternate variant encodes the lower activity form found in most other tissues.
Immunogen
KHK antibody was raised in mouse using a full length recombinant protein of human KHK (NP_000212) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-KHK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KHK antibody
Mouse monoclonal KHK antibody
Product Categories/Family for anti-KHK antibody
Proteases, Inhibitors, & Enzymes
Applications Tested/Suitable for anti-KHK antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-KHK antibody
FC: 1:100
IF: 1:100
WB: 1:2000
Western Blot (WB) of anti-KHK antibody
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant KHK protein (Right) or empty vector (Left) detected with KHK antibody
Immunofluorescence (IF) of anti-KHK antibody
Immunofluorescent staining of COS7 cells transiently transfected with recombinant KHK protein using KHK antibody
NCBI/Uniprot data below describe general gene information for KHK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000212
[Other Products]
NCBI GenBank Nucleotide #
NM_000221.2
[Other Products]
UniProt Primary Accession #
P50053
[Other Products]
UniProt Secondary Accession #
Q6IBK2; Q99532; Q9BRJ3; Q9UMN1[Other Products]
UniProt Related Accession #
P50053[Other Products]
Molecular Weight
32,730 Da
NCBI Official Full Name
ketohexokinase isoform a
NCBI Official Synonym Full Names
ketohexokinase (fructokinase)
NCBI Official Symbol
KHK [Similar Products]
NCBI Protein Information
ketohexokinase
UniProt Protein Name
Ketohexokinase
UniProt Synonym Protein Names
Hepatic fructokinase
Protein Family
Ketohexokinase
UniProt Gene Name
KHK [Similar Products]
UniProt Entry Name
KHK_HUMAN
NCBI Summary for KHK
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for KHK
KHK iso2: Defects in KHK are the cause of fructosuria (FRUCT). Benign defect of intermediary metabolism. Belongs to the carbohydrate kinase PfkB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.3; Carbohydrate Metabolism - fructose and mannose; Transferase
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: ketohexokinase activity; ATP binding
Biological Process: carbohydrate metabolic process; carbohydrate phosphorylation; pathogenesis; fructose catabolic process
Disease: Fructosuria, Essential
Research Articles on KHK
1. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
