Product Name
ACPP, Recombinant Protein
Full Product Name
ACPP, 33-386aa, Human, His-tag, Baculovirus (Bioactivity Validated)
Product Synonym Names
Prostatic acid phosphatase isoform PAP; ACPP; 5-NT; ACP-3; ACP3; 5-NT; 5-nucleotidase Acid phosphatase prostate; ACP 3; ACP3; acpP Ecto-5-nucleotidase; PAP; PPAP_HUMAN Prostatic acid phosphatase; Thiamine monophosphatase; TMPase
Product Gene Name
ACPP recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
KELKFVTLVF RHGDRSPIDT FPTDPIKESS WPQGFGQLTQ LGMEQHYELG EYIRKRYRKF LNESYKHEQV YIRSTDVDRT LMSAMTNLAA LFPPEGVSIW NPILLWQPIP VHTVPLSEDQ LLYLPFRNCP RFQELESETL KSEEFQKRLH PYKDFIATLG KLSGLHGQDL FGIWSKVYDP LYCESVHNFT LPSWATEDTM TKLRELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILNHMK RATQIPSYKK LIMYSAHDTT VSGLQMALDV YNGLLPPYAS CHLTELYFEK GEYFVEMYYR NETQHEPYPL MLPGCSPSCP LERFAELVGP VIPQDWSTEC MTTNSHQGTE DSTDHHHHHH
3D Structure
ModBase 3D Structure for P15309
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 100,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of ACPP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACPP recombinant protein
ACPP, also known as prostatic acid phosphatase isoform PAP, is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Recombinant human ACPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for ACPP recombinant protein
Cancer; Enzymes & Proteases
Applications Tested/Suitable for ACPP recombinant protein
SDS-PAGE
SDS-Page of ACPP recombinant protein
NCBI/Uniprot data below describe general gene information for ACPP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001090
[Other Products]
NCBI GenBank Nucleotide #
NM_001099.4
[Other Products]
UniProt Primary Accession #
P15309
[Other Products]
UniProt Secondary Accession #
Q5FBY0; Q96KY0; Q96QK9; Q96QM0; D3DNC6[Other Products]
UniProt Related Accession #
P15309[Other Products]
Molecular Weight
41.8kDa (360aa) 40-57KDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
prostatic acid phosphatase isoform PAP
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
ACPP [Similar Products]
NCBI Official Synonym Symbols
ACP3; 5'-NT; ACP-3
[Similar Products]
NCBI Protein Information
prostatic acid phosphatase
UniProt Protein Name
Prostatic acid phosphatase
UniProt Synonym Protein Names
5'-nucleotidase (EC:3.1.3.5); 5'-NT
Protein Family
Acyl carrier protein
UniProt Gene Name
ACPP [Similar Products]
UniProt Synonym Gene Names
PAP; 5'-NT; TMPase [Similar Products]
NCBI Summary for ACPP
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
UniProt Comments for ACPP
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Product References and Citations for ACPP recombinant protein
Chuang TD., et al. (2010) J Biol Chem. 285:23598-23606.; Taira A., et al. (2007) Oncology. 21:1003-1010.;
Research Articles on ACPP
1. Enhanced TDPase and TMPase activities may contribute to the reduction of TDP level in AD patients. The results imply that an imbalance of phosphorylation-dephosphorylation related to thiamine and glucose metabolism may be a potential target for AD prevention and therapy.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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