CLC3, Polyclonal Antibody

Anti -CLC3 (Clcn3, Chloride Channel)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 4; NC_000004.11 (170541722..170642157). Location: 4q33

Polyclonal

IgG

Rabbit

Recognizes rat CLC3. No significant sequence homology is detected with other CLCs.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CLCN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains. Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. Rat CLC-3 is 760aa membrane protein (human CLC-3 760 or 820aa; mouse CLC-3 760/818aa) (1). Alternatively spliced short forms of mouse and human CLC-3 have also been found. CLC-3 is abundant in brain (olfactory, hippocampus, and cerebellum) and moderate levels are found in the lung, kidney, and adrenals. CLC-3 has been implicated in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.

Antibodies; Abs to Ion Channel

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.

90,966 Da[Similar Products]

chloride channel 3

H(+)/Cl(-) exchange transporter 3; H(+)/Cl(-) exchange transporter 3; OTTHUMP00000219460; OTTHUMP00000219503; OTTHUMP00000219505; OTTHUMP00000219506; chloride channel protein 3; chloride transporter ClC-3

H(+)/Cl(-) exchange transporter 3

CLCN3_HUMAN

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

CLCN3: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Transporter; Transporter, ion channel; Membrane protein, multi-pass; Channel, chloride; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: Golgi apparatus; specific granule; cell surface; late endosome membrane; early endosome; integral to membrane; vesicle membrane; phagocytic vesicle; secretory granule; Golgi membrane; synaptic vesicle; membrane; early endosome membrane; apical plasma membrane; late endosome; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: protein binding; chloride channel activity; protein homodimerization activity; protein heterodimerization activity; voltage-gated chloride channel activity; ATP binding; antiporter activity; PDZ domain binding

Biological Process: transport; regulation of pH; negative regulation of cell volume; transmembrane transport; endosomal lumen acidification

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