HMGA2, Polyclonal Antibody

High mobility group protein HMGI-C; High mobility group AT-hook protein 2; HMGA2; HMGIC

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This HMGA2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 84-118 amino acids from the C-terminal region of human HMGA2.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-HMGA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.

Crown Antibodies; Cancer; Developmental Biology; Signal Transduction; Stem Cells

Western Blot (WB), ELISA (EIA)

WB~~1:2000

All lanes : Anti-HMGA2 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: NIH/3T3 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 12 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

11832

high mobility group AT-hook 2

high mobility group protein HMGI-C

High mobility group protein HMGI-C

HMGIC  [Similar Products]

HMGA2_HUMAN

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nucleoplasm; nuclear chromosome; nucleus

Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; nucleosomal DNA binding; DNA binding; AT DNA binding; DNA-dependent protein kinase activity; SMAD binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding; DNA bending activity

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; mesodermal cell differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; base-excision repair; regulation of growth; chondrocyte differentiation; negative regulation of retroviral genome replication; mitosis; transcription, DNA-dependent; response to virus; heterochromatin formation; DNA damage response, detection of DNA damage; stem cell differentiation; DNA catabolic process, endonucleolytic; negative regulation of DNA binding; chromosome condensation; mesenchymal cell differentiation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; chromosome breakage; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Leiomyoma, Uterine

Patel U.A.,et al.Biochem. Biophys. Res. Commun. 201:63-70(1994).
Schoenmakers E.F.P.M.,et al.Nat. Genet. 10:436-444(1995).
Chau K.-Y.,et al.Nucleic Acids Res. 23:4262-4266(1995).
Ashar H.R.,et al.Genomics 31:207-214(1996).
Hauke S.,et al.Biochim. Biophys. Acta 1729:24-31(2005).

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