BAFF, ELISA Kit

Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor (ligand) superfamily, member 13b; B lymphocyte stimulator; B-cell-activating factor; BAFF; BLyS; CD257; Dendritic cell-derived TNF-like molecule; soluble form; TALL-1; TN13B_HUMAN; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF13B; Tumor necrosis factor ligand superfamily member 13b

For Research Use Only. Not for use in diagnostic procedures.

Natural and recombinant human BAFF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of TNFSF13B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS175962 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BAFF (TNFSF13B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNFSF13B. The ELISA analytical biochemical technique of the MBS175962 kit is based on TNFSF13B antibody-TNFSF13B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFSF13B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFSF13B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: human BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BAFF has been precoated onto 96-well plates. Standards(NSO, A134-L285) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BAFF amount of sample captured in plate.
Background: BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas.1 The TNF superfamily member B cell-activating factor (BAFF) plays an important role inhumoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligandthat promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R).3Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble humanBAFF (A134-L295) with the molecular mass of 19.6KDa.

Human BAFF PicoKine ELISA Kit standard curve

17,578 Da

tumor necrosis factor (ligand) superfamily, member 13b

tumor necrosis factor ligand superfamily member 13B; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20

Tumor necrosis factor ligand superfamily member 13B

BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1  [Similar Products]

TN13B_HUMAN

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell cycle regulation; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 13q32-q34

Cellular Component: extracellular space; perinuclear region of cytoplasm; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; B cell costimulation; B cell homeostasis; immunoglobulin secretion; T cell costimulation; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of germinal center formation; immune response; positive regulation of T cell proliferation; signal transduction

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