ABL2, Polyclonal Antibody

ABL2; ABL 2; ABL-2; ABLL; ARG; Abelson-related gene protein; Tyrosine-protein kinase ARG; P42684; Abelson tyrosine-protein kinase 2; ABL proto-oncogene 2, non-receptor tyrosine kinase

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ABL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit IgG polyclonal antibody for Abelson tyrosine-protein kinase 2 (ABL2) detection.
Background: Tyrosine-protein kinase ABL2, also known as Abelson-related gene (Arg), is an enzyme that in humans is encoded by the ABL2 gene. This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western blot analysis of ABL2 expression in mouse brain extract (lane 1) and HELA whole cell lysates (lane 2). ABL2 at 140KD was detected using rabbit anti- ABL2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

ABL2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- ABL2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.

60,864 Da

ABL proto-oncogene 2, non-receptor tyrosine kinase

Abelson tyrosine-protein kinase 2

Abelson tyrosine-protein kinase 2

ABLL; ARG  [Similar Products]

ABL2_HUMAN

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; Oncoprotein; EC 2.7.10.2; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: actin cytoskeleton; cytosol; extrinsic to internal side of plasma membrane

Molecular Function: actin filament binding; actin monomer binding; magnesium ion binding; manganese ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein-tyrosine kinase activity; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; innate immune response; peptidyl-tyrosine phosphorylation; positive regulation of oxidoreductase activity; protein modification process; regulation of autophagy; regulation of cell adhesion; regulation of cell proliferation; regulation of endocytosis; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

1. "Entrez Gene: ABL2 v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)".
2. Cao C, Leng Y, Li C, Kufe D (April 2003). "Functional interaction between the c-Abl and Arg protein-tyrosine kinases in the oxidative stress response". J. Biol. Chem. 278 (15): 12961-7.
3. Kruh GD, King CR, Kraus MH, Popescu NC, Amsbaugh SC, McBride WO, Aaronson SA(Jan 1987). "A novel human gene closely related to the abl proto-oncogene". Science234 (4783): 1545-8.

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