BMP-7, Monoclonal Antibody

Anti-Human BMP-7

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Purified

At -20 degree C

Small volumes of anti-BMP-7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Produced in mice using highly pure (>98%) recombinant human BMP-7/OP-1 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.

Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant carcinoma. The recommended concentration is 5.0 ug/mL with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Information and photo are courtesy of Flagship Biosciences, LLC - Histology Services.

Sandwich ELISA: In a sandwich ELISA (assuming 100 ul/well), when used as a capture antibody at a concentration of 2.0-4.0 ug/ml, this antibody will detect at least 100 pg/ml of recombinant human BMP-7/OP-1 when used with biotinylated antigen-affinity purified anti-hBMP-7/OP-1 as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

49,313 Da

bone morphogenetic protein 7

bone morphogenetic protein 7; osteogenic protein 1

Bone morphogenetic protein 7

OP1; BMP-7; OP-1  [Similar Products]

BMP7_HUMAN

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: response to peptide hormone stimulus; negative regulation of MAP kinase activity; axon guidance; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; BMP signaling pathway; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; branching morphogenesis of a tube; mesoderm formation; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; epithelial to mesenchymal transition; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; neurite morphogenesis; growth

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