aggrecan, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 7; NC_000073.6 (79053483..79115099). Location: 7 D3; 7 39.0 cM

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

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Small volumes of ACAN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9323417 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the aggrecan (ACAN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACAN. The ELISA analytical biochemical technique of the MBS9323417 kit is based on ACAN antibody-ACAN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACAN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACAN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ACAN (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

221,941 Da

aggrecan

aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein

Aggrecan core protein

Agc; Agc1; CSPCP  [Similar Products]

PGCA_MOUSE

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Extracellular matrix; Secreted, signal peptide; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: protein binding; extracellular matrix structural constituent; metal ion binding; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: central nervous system development; collagen fibril organization; proteoglycan biosynthetic process; chondrocyte development; cell adhesion; negative regulation of cell migration; cartilage condensation

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