Ankyrin-2 (ANK2), ELISA Kit

DKFZp686M09125; DKFZp686P0948; FLJ38277; LQT4; ankyrin 2; ankyrin B; ankyrin; brain; ankyrin; nonerythroid; ankyrin-2; nonerythrocytic; brank-2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse ANK2. No significant cross-reactivity or interference between Mouse ANK2 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of ANK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2800246 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ankyrin-2 (ANK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANK2. The ELISA analytical biochemical technique of the MBS2800246 kit is based on ANK2 antibody-ANK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

This assay employs a two-site sandwich ELISA to quantitate ANK2 in samples. An antibody specific for ANK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

45,089 Da

ankyrin 2, brain

ankyrin-2

Ankyrin-2

ANK-2  [Similar Products]

Attaches integral membrane proteins to cytoskeletal elements. Also binds to cytoskeletal proteins. Required for coordinate assembly of Na/Ca exchanger, Na/K ATPase and InsP3 receptor at sarcoplasmic reticulum sites in cardiomyocytes. Required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) in the inner segment of rod photoreceptors (PubMed:19007774). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).

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