ARID5B, Polyclonal Antibody

Anti-ARID5B Antibody (aa500-550) IHC-plus; ARID5B; DESRT; Modulator recognition factor 2; MRF2; MRF-2; MRF1-like protein; Human ARID5B

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Human ARID5B

Protein A Purified

PBS, 0.05% BSA, 0.05% sodium azide

0.5 mg/ml (lot specific)

Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Small volumes of anti-ARID5B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

IHC-P (10 ug/ml), WB (2 - 4 ug/ml)

Anti-ARID5B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

Western blot of MRF2 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Western blot of MRF2 in human U87 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate using at 3 ug/ml, 3 ug/ml and 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

36,282 Da

AT rich interactive domain 5B (MRF1-like)

AT-rich interactive domain-containing protein 5B; MRF1-like protein; ARID domain-containing protein 5B; modulator recognition factor 2 (MRF2)

AT-rich interactive domain-containing protein 5B

DESRT; MRF2; ARID domain-containing protein 5B; MRF-2  [Similar Products]

ARI5B_HUMAN

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Mrf-2: Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population. Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL). ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes. Belongs to the ARID5B family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; adrenal gland development; multicellular organism growth; male gonad development; platelet-derived growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; palate development; liver development; post-embryonic development; regulation of transcription from RNA polymerase II promoter; skeletal morphogenesis; positive regulation of transcription factor activity; kidney development; negative regulation of transcription, DNA-dependent; cell development; muscle morphogenesis; female gonad development

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