AT-rich interactive domain-containing protein 5B (ARID5B), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ARID5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7205303 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AT-rich interactive domain-containing protein 5B (ARID5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ARID5B. The ELISA analytical biochemical technique of the MBS7205303 kit is based on ARID5B antibody-ARID5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARID5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARID5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

31,400 Da

AT rich interactive domain 5B (MRF1-like)

AT-rich interactive domain-containing protein 5B; MRF-2; ARID class DNA binding protein; modulator recognition factor 2; ARID domain-containing protein 5B; modulator recognition factor protein 2; AT rich interactive domain 5B (Mrf1 like); developmentally and sexually retarded with transient immune abnormalities protein

AT-rich interactive domain-containing protein 5B

Desrt; Mrf2; ARID domain-containing protein 5B; Desrt; MRF-2  [Similar Products]

ARI5B_MOUSE

Mrf-2: Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population. Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL). ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes. Belongs to the ARID5B family. 3 isoforms of the human protein are produced by alternative splicing.

Cellular Component: nucleus

Molecular Function: DNA binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; transcription, DNA-dependent; adrenal gland development; multicellular organism growth; male gonad development; platelet-derived growth factor receptor signaling pathway; palate development; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; regulation of transcription from RNA polymerase II promoter; nitrogen compound metabolic process; regulation of transcription, DNA-dependent; skeletal morphogenesis; positive regulation of transcription factor activity; kidney development; muscle morphogenesis; cell development; female gonad development

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