Product Name
B3GNT2, siRNA
Full Product Name
B3GNT2 siRNA (Human)
Product Synonym Names
B3GALT7; B3GNT1; UDP-GlcNAc:betaGal beta-1.3-N-acetylglucosaminyltransferase 2; BGnT-2; Beta-1.3-Gn-T2; Beta-1.3-N-acetylglucosaminyltransferase 2; Beta3Gn-T2; Beta-1.3-N-acetylglucosaminyltransferase 1; BGnT-1; Beta-1.3-Gn-T1; Beta3Gn-T1; Beta-1.3-galactosyltransferase 7; Beta-1.3-GalTase 7; Beta3Gal-T7; Beta3GalT7; b3Gal-T7; Beta-3-Gx-T7; UDP-Gal:beta-GlcNAc beta-1.3-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1.3-galactosyltransferase 7
Product Gene Name
B3GNT2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NY97
Specificity
B3GNT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human B3GNT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of B3GNT2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
B3GNT2 sirna
siRNA to inhibit B3GNT2 expression using RNA interference
Applications Tested/Suitable for B3GNT2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for B3GNT2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006568.2
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NCBI GenBank Nucleotide #
NM_006577.5
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UniProt Primary Accession #
Q9NY97
[Other Products]
UniProt Secondary Accession #
Q54AC1; Q9NQQ9; Q9NQR0; Q9NUT9[Other Products]
UniProt Related Accession #
Q9NY97[Other Products]
Molecular Weight
45,484 Da
NCBI Official Full Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
NCBI Official Symbol
B3GNT2 [Similar Products]
NCBI Official Synonym Symbols
B3GNT; BGNT2; B3GNT1; BGnT-2; B3GN-T2; B3GNT-2; BETA3GNT
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NCBI Protein Information
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
UniProt Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
UniProt Synonym Protein Names
Beta-1,3-N-acetylglucosaminyltransferase 1; BGnT-1; Beta-1,3-Gn-T1; Beta3Gn-T1; Beta-1,3-galactosyltransferase 7; Beta-1,3-GalTase 7; Beta3Gal-T7; Beta3GalT7; b3Gal-T7; Beta-3-Gx-T7; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2; BGnT-2; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 2; Beta3Gn-T2; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7
Protein Family
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
UniProt Gene Name
B3GNT2 [Similar Products]
UniProt Synonym Gene Names
B3GALT7; B3GNT1; BGnT-1; Beta-1,3-Gn-T1; Beta3Gn-T1; Beta-1,3-GalTase 7; Beta3Gal-T7; Beta3GalT7; b3Gal-T7; BGnT-2; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 2; Beta3Gn-T2 [Similar Products]
UniProt Entry Name
B3GN2_HUMAN
NCBI Summary for B3GNT2
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. [provided by RefSeq, Jul 2008]
UniProt Comments for B3GNT2
B3GNT2: Catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Glycan Metabolism - keratan sulfate biosynthesis; Motility/polarity/chemotaxis; Cell development/differentiation; EC 2.4.1.-; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase
Chromosomal Location of Human Ortholog: 2p15
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity
Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; axon guidance; poly-N-acetyllactosamine biosynthetic process; cellular protein metabolic process; O-glycan processing; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; sensory perception of smell; pathogenesis; post-translational protein modification
Research Articles on B3GNT2
1. data reveals beta3GnT2 and polyLacNAc may be involved in the progression of the pre-malignant lesions of human the uterine cervix.
Precautions
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