FOXC1/2, Polyclonal Antibody

FOXC1; FKHL7; FREAC3; Forkhead box protein C1; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FREAC-3; FOXC2; FKHL14; MFH1; Forkhead box protein C2; Forkhead-related protein FKHL14; Mesenchyme fork head protein 1; MFH-1 protein; Transcription factor FKH-14

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of FOXC1/2 protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-FOXC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to FOXC1/2

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)

Western blot analysis of FOXC1/2 expression in THP1 (A), RAW264.7 (B) whole cell lysates.

Immunohistochemical analysis of FOXC1/2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of FOXC1/2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

56,789 Da

forkhead box C1

forkhead box protein C1

Forkhead box protein C1

FKHL7; FREAC3; FREAC-3  [Similar Products]

FOXC1_HUMAN

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.

Protein type: DNA-binding; Cell cycle regulation; Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; DNA bending activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; glycosaminoglycan metabolic process; neural crest cell development; paraxial mesoderm formation; positive regulation of transcription, DNA-dependent; heart development; cardiac muscle cell proliferation; ovarian follicle development; regulation of transcription, DNA-dependent; ureteric bud development; germ cell migration; ventricular cardiac muscle morphogenesis; negative regulation of mitotic cell cycle; lacrimal gland development; regulation of blood vessel size; skeletal development; mesenchymal cell development; somitogenesis; Notch signaling pathway; ossification; cell migration; camera-type eye development; in utero embryonic development; regulation of organ growth; odontogenesis of dentine-containing teeth; cell proliferation; eye development; artery morphogenesis; embryonic heart tube development; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; brain development; lymph vessel development; vascular endothelial growth factor receptor signaling pathway

Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1

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