AQP2, Polyclonal Antibody

AQP2; AQP-CD; WCH-CD; Aquaporin-2; ADH water channel; AQP-2; aquaporin 2 (collecting duct)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.

AQP2 antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

AQP2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Small volumes of anti-AQP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

AQP2 antibody can be used for detection of AQP2 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western blot analysis of AQP2 in A431 cell lysate with AQP2 antibody at 1 μg/ml.

Immunohistochemistry of AQP2 in human kidney tissue with AQP2 antibody at 5 μg/mL.

Immunofluorescence of AQP2 in human kidney tissue with AQP2 antibody at 20 μg/mL.

Predicted: 30 kDa

Observed: 28 kDa

aquaporin 2 (collecting duct)

aquaporin-2; AQP-2; aquaporin-CD; ADH water channel; water-channel aquaporin 2; collecting duct water channel protein; water channel protein for renal collecting duct

Aquaporin-2

AQP-2; AQP-CD  [Similar Products]

AQP2_HUMAN

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Transporter, aquaporin family; Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: recycling endosome; protein complex; membrane; rough endoplasmic reticulum; clathrin-coated vesicle; integral to plasma membrane; basolateral plasma membrane; lysosome; apical plasma membrane; early endosome; plasma membrane; trans-Golgi network

Molecular Function: water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; PDZ domain binding; glycerol channel activity

Biological Process: actin filament depolymerization; glycerol transport; response to glucagon stimulus; cellular water homeostasis; apoptosis; response to lithium ion; water transport; cellular response to water deprivation; response to lipopolysaccharide; female pregnancy; excretion; response to salt stress; response to starvation; hyperosmotic response; renal water transport; renal water homeostasis; response to calcium ion; positive regulation of calcium ion transport; transmembrane transport; aging

Disease: Diabetes Insipidus, Nephrogenic, Autosomal

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