Product Name
Calnexin-CT (CNX), Polyclonal Antibody
Full Product Name
Calnexin-CT Antibody: Dylight 633
Product Synonym Names
Rabbit Anti-Dog Calnexin-CT Polyclonal; Calnexin antibody; CALX_HUMAN antibody; CANX antibody; CNX antibody; FLJ26570 antibody; Histocompatibility complex class I antigen binding protein p88 antibody; IP90 antibody; Major histocompatibility complex class I antigen-binding protein p88 antibody; P90 antibody; Calnexin
Product Gene Name
anti-CNX antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: Calnexin-CT (MBS806306)
Dylight 633 Conjugated Antibody: Calnexin-CT (MBS8004330)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P24643
Species Reactivity
Human, Mouse, Rat, Bovine, Monkey, Chicken, Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Fruit Fly, Xenopus
Specificity
Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
Purity/Purification
Protein A Purified
Form/Format
PBS pH 7.2, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Cellular Localization
Endoplasmic Reticulum; Endoplasmic Reticulum Membrane; Melanosome
Certificate of Analysis
A 1:2000 dilution was sufficient for detection of Calnexin in 10 ug of HeLa cell lysate by ECL immunoblot analysis.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-CNX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CNX antibody
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Categories/Family for anti-CNX antibody
Cell Signaling; Tags and Cell Markers; Organelle Markers
Applications Tested/Suitable for anti-CNX antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-CNX antibody
WB: 1:2000
ICC/IF: 1:100
IHC: 1:100
Immunocytochemistry (ICC) of anti-CNX antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody. Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at-20C for 10 minutes. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody at 1:80 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.
NCBI/Uniprot data below describe general gene information for CNX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001003232.1
[Other Products]
NCBI GenBank Nucleotide #
NP_001003232.1
[Other Products]
UniProt Primary Accession #
P24643
[Other Products]
UniProt Related Accession #
P24643[Other Products]
NCBI Official Full Name
calnexin
NCBI Official Synonym Full Names
calnexin
NCBI Official Symbol
CANX [Similar Products]
NCBI Official Synonym Symbols
PP90
[Similar Products]
NCBI Protein Information
calnexin
UniProt Protein Name
Calnexin
UniProt Synonym Protein Names
pp90
UniProt Gene Name
CANX [Similar Products]
UniProt Entry Name
CALX_CANFA
Product References and Citations for anti-CNX antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
Research Articles on CNX
1. Calnexin shuttles between the rough ER and the mitochondria-associated membrane depending on its palmitoylation status.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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