Centromere protein J (CENPJ), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CENPJ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7240959 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Centromere protein J (CENPJ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CENPJ. The ELISA analytical biochemical technique of the MBS7240959 kit is based on CENPJ antibody-CENPJ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CENPJ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CENPJ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

153,000 Da[Similar Products]

centromere protein J

centromere protein J; LAG-3-associated protein; LYST-interacting protein 1; LYST-interacting protein LIP1; LYST-interacting protein LIP7; centrosomal P4.1-associated protein

Centromere protein J

CPAP; LAP; LIP1; CENP-J  [Similar Products]

CENPJ_HUMAN

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

CENPJ: Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Defects in CENPJ are the cause of microcephaly primary type 6 (MCPH6). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. Defects in CENPJ are the cause of Seckel syndrome type 4 (SCKL4). SCKL4 is a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation. Belongs to the TCP10 family.

Protein type: Cell cycle regulation; Microtubule-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13q12.12

Cellular Component: centriole; microtubule; centrosome; cytosol; gamma-tubulin small complex

Molecular Function: tubulin binding; protein domain specific binding; protein binding; protein kinase binding

Biological Process: regulation of centriole replication; cell division; organelle organization and biogenesis; centriole replication; mitotic cell cycle; microtubule nucleation; G2/M transition of mitotic cell cycle; microtubule polymerization

Disease: Microcephaly 6, Primary, Autosomal Recessive; Seckel Syndrome 4

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