Full Product Name
Rabbit Polyclonal to Human CD2AP
Product Synonym Names
Anti-CD2AP Antibody IHC-plus; CD2AP; Adapter protein CMS; CMS; CD2-associated protein; Human CD2AP
Product Gene Name
anti-CD2AP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y5K6
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purified
Form/Format
PBS, pH 7.4, 0.02% sodium azide
Concentration
1 mg/ml (lot specific)
Immunogen Description
Synthetic peptide
Immunogen Type
Synthetic peptide
Preparation and Storage
Store at -20 degree C for up to one year.
Other Notes
Small volumes of anti-CD2AP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD2AP antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD2AP antibody
ELISA (1:5000 - 1:10000), IHC-P (5 ug/ml), WB (1:500 - 1:2000)
Immunohistochemistry (IHC) of anti-CD2AP antibody
Anti-CD2AP antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NCBI/Uniprot data below describe general gene information for CD2AP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036252.1
[Other Products]
NCBI GenBank Nucleotide #
NM_012120.2
[Other Products]
UniProt Primary Accession #
Q9Y5K6
[Other Products]
UniProt Secondary Accession #
Q5VYA3; Q9UG97; A6NL34[Other Products]
UniProt Related Accession #
Q9Y5K6[Other Products]
Molecular Weight
71,451 Da
NCBI Official Full Name
CD2-associated protein
NCBI Official Synonym Full Names
CD2-associated protein
NCBI Official Symbol
CD2AP [Similar Products]
NCBI Official Synonym Symbols
CMS
[Similar Products]
NCBI Protein Information
CD2-associated protein; adapter protein CMS; cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains
UniProt Protein Name
CD2-associated protein
UniProt Synonym Protein Names
Adapter protein CMS; Cas ligand with multiple SH3 domains
Protein Family
CD2-associated protein
UniProt Gene Name
CD2AP [Similar Products]
UniProt Entry Name
CD2AP_HUMAN
NCBI Summary for CD2AP
This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin s, SH3 domains, and a proline-rich region containing s for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008]
UniProt Comments for CD2AP
CD2AP: an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Required for cytokinesis. Links CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. Attaches to membrane proteins via its three SH3 domains and attaches to and rearranges the cytoskeleton via its C-terminus. CD2AP?s interactions with the cytoskeleton result in receptor clustering and endocysosis as well as targeting proteins to degradative vesicles in the kidney. Mice carrying a heterozygous knockout of CD2AP show defects in kidney filtration, and mutations in the human gene are associated with focal segmental glomerulosclerosis (FSGS3).
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: filamentous actin; ruffle; cytoplasm; plasma membrane; nucleolus; intercellular junction; actin cytoskeleton
Molecular Function: protein binding; structural constituent of cytoskeleton; SH3 domain binding
Biological Process: mitosis; cell division; protein complex assembly; signal transduction; substrate-bound cell migration, cell extension; negative regulation of transforming growth factor-beta1 production
Disease: Focal Segmental Glomerulosclerosis 3, Susceptibility To
Research Articles on CD2AP
1. CD2AP gene variants may contribute to susceptibility to end-stage renal disease in patients with type 1 diabetes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
