ADAM metallopeptidase with thrombospondin type 1 motif, 5, ELISA Kit

Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAM metallopeptidase with thrombospondin type 1 motif; 5

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse ADAMTS5. No significant cross-reactivity or interference between mouse ADAMTS5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADAMTS5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS901189 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS5. The ELISA analytical biochemical technique of the MBS901189 kit is based on ADAMTS5 antibody-ADAMTS5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

101,844 Da

a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)

A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAMTS-5; ADAM-TS 5; implantin; aggrecanase-2

A disintegrin and metalloproteinase with thrombospondin motifs 5

ADAM-TS 5; ADAM-TS5; ADAMTS-5  [Similar Products]

ATS5_MOUSE

ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Protein type: Secreted, signal peptide; Extracellular matrix; EC 3.4.24.-; Secreted; Motility/polarity/chemotaxis; Protease

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; metallopeptidase activity; zinc ion binding; hydrolase activity; metal ion binding; metalloendopeptidase activity

Biological Process: defense response to bacterium; proteolysis

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