PER2, Polyclonal Antibody

KIAA0347; Period circadian protein homolog 2; hPER2; Circadian clock protein PERIOD 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of PER2 (pS662) protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-PER2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to PER2 (pS662)

Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)

Western blot analysis of PER2 (pS662) expression in NIH3T3 (A), mouse lung (B) whole cell lysates.

Immunohistochemical analysis of PER2 (pS662) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

45,175 Da

period circadian clock 2

period circadian protein homolog 2

Period circadian protein homolog 2

KIAA0347; hPER2  [Similar Products]

PER2_HUMAN

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]

PER2: a transcription factor belonging to the basic helix-loop-helix (bHLH) family. A member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hr rhythm in the suprachiasmatic nucleus and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between per1 and per2 oscillations. The expression rhythms appear to originate from retina.

Protein type: Nucleolus; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: protein binding; transcription coactivator activity; ubiquitin binding

Biological Process: circadian regulation of gene expression; circadian rhythm; fatty acid metabolic process; gluconeogenesis; glycogen biosynthetic process; lactate biosynthetic process; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of cell cycle; regulation of circadian rhythm; regulation of glutamate uptake during transmission of nerve impulse; regulation of insulin secretion; regulation of neurogenesis; regulation of vasoconstriction; transcription, DNA-dependent; white fat cell differentiation

Disease: Advanced Sleep Phase Syndrome, Familial, 1

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