Product Name
MASP1, siRNA
Full Product Name
MASP1 siRNA (Mouse)
Product Synonym Names
CRARF; MASP3; Mannan-binding lectin serine protease 1; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1; MASP-1; Mannose-binding protein-associated serine protease; Ra-reactive factor serine protease p100; RaRF; Serine protease 5
Product Gene Name
MASP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P98064
Specificity
MASP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MASP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MASP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MASP1 sirna
siRNA to inhibit MASP1 expression using RNA interference
Applications Tested/Suitable for MASP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MASP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032581.2
[Other Products]
NCBI GenBank Nucleotide #
NM_008555.2
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UniProt Primary Accession #
P98064
[Other Products]
UniProt Secondary Accession #
Q8CD27; Q8CIR8; Q920S0; A2RRH8; A2RRH9[Other Products]
UniProt Related Accession #
P98064[Other Products]
Molecular Weight
82,446 Da
NCBI Official Full Name
mannan-binding lectin serine protease 1
NCBI Official Synonym Full Names
mannan-binding lectin serine peptidase 1
NCBI Official Symbol
Masp1 [Similar Products]
NCBI Official Synonym Symbols
CCPII; Crarf; Masp1/3; AW048060
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NCBI Protein Information
mannan-binding lectin serine protease 1
UniProt Protein Name
Mannan-binding lectin serine protease 1
UniProt Synonym Protein Names
Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1; MASP-1
Protein Family
Mannan-binding lectin serine protease
UniProt Gene Name
Masp1 [Similar Products]
UniProt Synonym Gene Names
Crarf; Masp3; MASP-1; RaRF [Similar Products]
UniProt Entry Name
MASP1_MOUSE
UniProt Comments for MASP1
MASP1: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Defects in MASP1 are the cause of 3MC syndrome type 1 (3MC1). 3MC1 is a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly arched eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.-; Protease
Cellular Component: extracellular space; extracellular region
Molecular Function: peptidase activity; protein homodimerization activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; metal ion binding; calcium ion binding; calcium-dependent protein binding; catalytic activity
Biological Process: immune system process; innate immune response; complement activation, lectin pathway; proteolysis; complement activation
Research Articles on MASP1
1. study provides conclusive evidence that an intact complement LP is essential to initiate CAIA, and that MAp44 may be an appropriate treatment for inflammatory arthritis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
