MASP1, Polyclonal Antibody

Mannan-binding lectin serine protease 1; 3421-; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1; MASP-1; Mannose-binding protein-associated serine protease; Ra-reactive factor serine protease p100; RaRF; Serine protease 5; Mannan-binding lectin serine protease 1 heavy chain; Mannan-binding lectin serine protease 1 light chain; MASP1; CRARF; CRARF1; PRSS5

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This MASP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-507 amino acids from the Central region of human MASP1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.32 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MASP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a serine protease that functions as a
component of the lectin pathway of complement activation. The
complement pathway plays an essential role in the innate and
adaptive immune response. The encoded protein is synthesized as a
zymogen and is activated when it complexes with the pathogen
recognition molecules of lectin pathway, the mannose-binding lectin
and the ficolins. This protein is not directly involved in
complement activation but may play a role as an amplifier of
complement activation by cleaving complement C2 or by activating
another complement serine protease, MASP-2. The encoded protein is
also able to cleave fibrinogen and factor XIII and may may be
involved in coagulation. A splice variant of this gene which lacks
the serine protease domain functions as an inhibitor of the
complement pathway. Alternate splicing results in multiple
transcript variants.

Immunology

Western Blot (WB), ELISA (EIA)

WB~~1:1000

MASP1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MASP1 antibody detected the MASP1 protein (arrow).

79247

mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)

mannan-binding lectin serine protease 1

Mannan-binding lectin serine protease 1

CRARF; CRARF1; PRSS5; MASP-1; RaRF  [Similar Products]

MASP1_HUMAN

This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

MASP1: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Defects in MASP1 are the cause of 3MC syndrome type 1 (3MC1). 3MC1 is a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly arched eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27-q28

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; protein binding; protein homodimerization activity; serine-type endopeptidase activity; calcium ion binding; calcium-dependent protein binding

Biological Process: receptor-mediated endocytosis; negative regulation of complement activation; innate immune response; proteolysis; complement activation, lectin pathway; complement activation

Disease: 3mc Syndrome 1

Kocsis, A., et al. J. Immunol. 185(7):4169-4178(2010)
Degn, S.E., et al. J. Immunol. Methods 361 (1-2), 37-50 (2010) :
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Skjoedt, M.O., et al. J. Biol. Chem. 285(11):8234-8243(2010)

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