Lactate Dehydrogenase A, Recombinant Protein

LDHA Mouse; L-lactate dehydrogenase A chain; LDH-A; LDH muscle subunit; LDH-M; Lactate Dehydrogenase A Mouse Recombinant

For Research Use Only. Not for use in diagnostic procedures.

Sf9, Baculovirus cells

Greater than 95.0% as determined by SDS-PAGE.

Sterile filtered colorless solution.
LDHA protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Small volumes of LDHA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: LDHA Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (1-332a.a.) and having a molecular mass of 37.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). LDHA is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction: LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

ENZYMES

36,499 Da

lactate dehydrogenase A

L-lactate dehydrogenase A chain

L-lactate dehydrogenase A chain

Ldh-1; Ldh1; LDH-A; LDH-M  [Similar Products]

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase

Chromosomal Location of Human Ortholog: 7 B3|7 30.6 cM

Cellular Component: cell-cell adherens junction; cytosol; membrane; mitochondrion; nucleus

Molecular Function: L-lactate dehydrogenase activity; lactate dehydrogenase activity

Biological Process: cellular response to extracellular stimulus; glucose catabolic process to lactate via pyruvate; lactate biosynthetic process from pyruvate

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