Cytochrome P450 17A1, Monoclonal Antibody

Purified Mouse Monoclonal Antibody (Mab); 20 lyase; CP17A_HUMAN; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 family 17; Cytochrome P450 family 17 subfamily A polypeptide 1; Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia; Cytochrome p450 XVIIA1; Cytochrome P450-C17; Cytochrome P450c17; OTTHUMP00000020382; P450 C17; P450c17; S17AH; Steroid 17 alpha hydroxylase/17,20 lyase; Steroid 17 alpha monooxygenase; Steroid 17-alpha-hydroxylase/17; Steroid 17-alpha-monooxygenase.

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Ascites

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CYP17A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Cancer; Cardiovascular; Metabolism; Signal Transduction

Western Blot (WB)

WB ~~ 1:1000

Western blot detection of Cytochrome P450 17A1 in Hela,HepG2 and A549 cell lysates using Cytochrome P450 17A1 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

57371

cytochrome P450, family 17, subfamily A, polypeptide 1

steroid 17-alpha-hydroxylase/17,20 lyase

Steroid 17-alpha-hydroxylase/17,20 lyase

CYP17; S17AH; Cytochrome P450c17  [Similar Products]

CP17A_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short ***** stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: EC 1.14.99.9; EC 4.1.2.30; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: endoplasmic reticulum membrane; mitochondrion; cell soma; axon; endoplasmic reticulum

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; steroid 17-alpha-monooxygenase activity; iron ion binding; heme binding; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; hormone biosynthetic process; progesterone metabolic process; androgen biosynthetic process; glucocorticoid biosynthetic process; sex differentiation; steroid biosynthetic process; sterol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency

Chung B.-C.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:407-411(1987). Picado-Leonard J.,et al.DNA 6:439-448(1987). Bradshaw K.D.,et al.Mol. Endocrinol. 1:348-354(1987). Brentano S.T.,et al.Mol. Endocrinol. 4:1972-1979(1990). Kagimoto M.,et al.Mol. Endocrinol. 2:564-570(1988).

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