Product Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (Plod1), Recombinant Protein
Full Product Name
Recombinant Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (Plod1)
Product Gene Name
Plod1 recombinant protein
[Similar Products]
Product Synonym Gene Name
Plod1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
19-728aa, Full Length of Mature Protein
Sequence
KDDAKLEDNL LVLTVATKET EGFRRFKRSA QFFNYKIQSL GLGEDWSVDG GPAAAGGGQK VRLLKKALEK HADKEDLVIL FVDSYDVVFA SGPRELLKKF QQAKSQVVFS AEEHIYPDRR LEAKYPTVPD GKRFLGSGGF IGYAPSLSKL VAEWEGQDSD SDQLFYTKIF LNPEKREQIN ISLDHRCRIF QNLDGALDEV VLKFEMGHVR ARNLAYDTLP VVVHGNGPTK LQLNYLGNYI PRFWTFETGC TVCDEGLRSL KGIGDEALPT VLVGVFIEQP TPFLSLFFLR LLRLRYPQKQ MRLFIHNQER HHKLQVEQFL AEHGSEYQSV KLVGPEVRMA NADARNMGAD LCRQDQTCTY YFSVDADVAL TEPNSLRLLI EQNKNVIAPL MTRHGRLWSN FWGGLSADGY YARSEDYVDI VQGRRVGVWN VPYISNIYLI KGSALRAELQ NVDLFHYSKL DSDMSFCANV RQQEVFMFLT NRHTFGHLLS LDNYQTTHLH NDLWEVFSNP EDWKEKYIHE NYTKALAGKL VETPCPDVYW FPIFTEAACD ELVEEMEHYG QWSLGDNKDN RIQGGYENVP TIDIHMNQIT FEREWHKFLV EYIAPMTEKL YPGYYTRAQF DLAFVVRYKP DEQPSLMPHH DASTFTVNIA LNRVGEDYEG GGCRFLRYNC SVRAPRKGWA LLHPGRLTHY HEGLPTTKGT RYIAVSFVDP
3D Structure
ModBase 3D Structure for Q9R0E2
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Plod1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Plod1 recombinant protein
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
NCBI/Uniprot data below describe general gene information for Plod1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035252.1
[Other Products]
NCBI GenBank Nucleotide #
NM_011122.3
[Other Products]
UniProt Primary Accession #
Q9R0E2
[Other Products]
UniProt Related Accession #
Q9R0E2[Other Products]
Molecular Weight
83,595 Da
NCBI Official Full Name
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
NCBI Official Synonym Full Names
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
NCBI Official Symbol
Plod1 [Similar Products]
NCBI Official Synonym Symbols
Lh1; Plod; AI854890; AV007929; 2410042F05Rik
[Similar Products]
NCBI Protein Information
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Synonym Protein Names
Lysyl hydroxylase 1; LH1
Protein Family
Procollagen-lysine,2-oxoglutarate 5-dioxygenase
UniProt Gene Name
Plod1 [Similar Products]
UniProt Synonym Gene Names
Plod; LH1 [Similar Products]
UniProt Comments for Plod1
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (PubMed:27119146).
Research Articles on Plod1
1. Plod1(-/-) mice offer an in vivo model for systematic analysis of the tissue-specific consequences of the lack of LH1 activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
