55 kDa erythrocyte membrane protein (MPP1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MPP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7242145 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 55 kDa erythrocyte membrane protein (MPP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPP1. The ELISA analytical biochemical technique of the MBS7242145 kit is based on MPP1 antibody-MPP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cardiovascular

49,854 Da

membrane protein, palmitoylated 1, 55kDa

55 kDa erythrocyte membrane protein; p55; aging-associated gene 12; migration-related gene 1; erythrocyte membrane protein p55; palmitoylated erythrocyte membrane protein

55 kDa erythrocyte membrane protein

DXS552E; EMP55; p55  [Similar Products]

EM55_HUMAN

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

EM55: Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity. Belongs to the MAGUK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cortical cytoskeleton; stereocilium; membrane; integral to plasma membrane; intracellular

Molecular Function: guanylate kinase activity; protein binding

Biological Process: signal transduction; nucleotide phosphorylation

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