PSTPIP1, Polyclonal Antibody

Anti -PSTPIP1, NT (PSTPIP1, CD2BP1, Proline-serine-threonine phosphatase-interacting protein 1, CD2-binding protein 1, H-PIP)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 15; NC_000015.9 (77287021..77329673). Location: 15q24-q25.1

Polyclonal

IgG

Rabbit

Human

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-PSTPIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome.

Antibodies; Abs to CD Markers

ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

47,591 Da[Similar Products]

proline-serine-threonine phosphatase interacting protein 1

proline-serine-threonine phosphatase-interacting protein 1; CD2-binding protein 1; CD2 antigen-binding protein 1; CD2 cytoplasmic tail-binding protein; PEST phosphatase-interacting protein 1

Proline-serine-threonine phosphatase-interacting protein 1

CD2BP1; PEST phosphatase-interacting protein 1  [Similar Products]

PPIP1_HUMAN

The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. [provided by RefSeq, Jul 2008]

Function: Involved in regulation of the actin cytoskeleton. May regulate the WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to the ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allows PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation

By similarity. Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Ref.1

Subunit structure: Interacts with PTPN18, ABL1, CD2AP and WAS

By similarity. Interacts with CD2, PTPN12 and MEFV/pyrin. Interacts with FASLG. Ref.1 Ref.6 Ref.7

Subcellular location: Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity. Cell projection › lamellipodium

By similarity. Cytoplasm › perinuclear region

By similarity. Cleavage furrow

By similarity. Note: Colocalized with the cortical actin cytoskeleton during interphase, lamellipodia and actin-rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact

By similarity. Ref.1

Tissue specificity: Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta. Ref.1 Ref.6

Domain: The coiled domain mediates interaction with PTPN18, PTPN12 and CD2AP. The SH3 domain mediates interaction with WAS and ABL1

By similarity. The SH3 and coiled-coil domains are necessary for the interaction with MEFV.

Post-translational modification: Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV

By similarity.

Involvement in disease: PAPA syndrome (PAPAS) [MIM:604416]: Characterized by autosomal dominant inheritance of early-onset, primarily affecting skin and joint tissues. Recurring inflammatory episodes lead to accumulation of sterile, pyogenic, neutrophil-rich material within the affected joints, ultimately resulting in significant destruction.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.6 Ref.9

Sequence similarities: Contains 1 FCH domain.Contains 1 SH3 domain.

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