Full Product Name
DIO1 Antibody (N-term)
Product Synonym Names
Type I iodothyronine deiodinase; 5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase; DIO1; ITDI1; TXDI1
Product Gene Name
anti-DIO1 antibody
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Antibody/Peptide Pairs
DIO1 peptide (MBS9226455) is used for blocking the activity of DIO1 antibody (MBS9202337)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
30-57
3D Structure
ModBase 3D Structure for P49895
Specificity
This DIO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-57 amino acids from the N-terminal region of human DIO1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.315 (lot specific)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-DIO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DIO1 antibody
DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD).
Product Categories/Family for anti-DIO1 antibody
Cancer; Metabolism; Neuroscience; Signal Transduction
Applications Tested/Suitable for anti-DIO1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-DIO1 antibody
WB~~1:1000
Western Blot (WB) of anti-DIO1 antibody
Western blot analysis of DIO1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DIO1 (arrow) was detected using the purified Pab.
NCBI/Uniprot data below describe general gene information for DIO1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000783.2
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NCBI Related Accession #
HumanNP_001034804.1; NP_001034805.1; NP_998758.1[Other Products]
NCBI GenBank Nucleotide #
NM_000792.5
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UniProt Primary Accession #
P49895
[Other Products]
UniProt Secondary Accession #
Q1RN02; Q3KNP8; Q6Q4C1; Q6Q4C2; Q6Q4C3; Q6Q4C4; Q6Q4C5; Q6Q4C6; Q6Q4C7; Q6Q4C9; Q8WWC6[Other Products]
UniProt Related Accession #
P49895[Other Products]
NCBI Official Full Name
type I iodothyronine deiodinase isoform a
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type I
NCBI Official Symbol
DIO1 [Similar Products]
NCBI Official Synonym Symbols
5DI; TXDI1
[Similar Products]
NCBI Protein Information
type I iodothyronine deiodinase
UniProt Protein Name
Type I iodothyronine deiodinase
UniProt Synonym Protein Names
5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase
Protein Family
Type I iodothyronine deiodinase
UniProt Gene Name
DIO1 [Similar Products]
UniProt Synonym Gene Names
ITDI1; TXDI1 [Similar Products]
UniProt Entry Name
IOD1_HUMAN
NCBI Summary for DIO1
The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq, Jul 2008]
UniProt Comments for DIO1
DIO1: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney. Belongs to the iodothyronine deiodinase family. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 1.97.1.10; Oxidoreductase
Chromosomal Location of Human Ortholog: 1p33-p32
Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane
Molecular Function: selenium binding; thyroxine 5'-deiodinase activity
Biological Process: thyroid hormone generation; hormone biosynthetic process
Product References and Citations for anti-DIO1 antibody
Landa,I., et.al., PLoS Genet. 5 (9), E1000637 (2009)
Research Articles on DIO1
1. The pattern of expression and role of triiodothyronine (T3) receptors and type I 5'-deiodinase in breast carcinomas, benign breast diseases, lactational change, and normal breast epithelium.
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