Full Product Name
DIO1 siRNA (Mouse)
Product Synonym Names
ITDI1; TXDI1; Type I iodothyronine deiodinase; 5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase
Product Gene Name
DIO1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61153
Specificity
DIO1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DIO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DIO1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DIO1 sirna
siRNA to inhibit DIO1 expression using RNA interference
Applications Tested/Suitable for DIO1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DIO1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031886.3
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NCBI GenBank Nucleotide #
NM_007860.3
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UniProt Primary Accession #
Q61153
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UniProt Secondary Accession #
Q05CY5; Q5FW63[Other Products]
UniProt Related Accession #
Q61153[Other Products]
Molecular Weight
29,568 Da
NCBI Official Full Name
type I iodothyronine deiodinase
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type I
NCBI Official Symbol
Dio1 [Similar Products]
NCBI Official Synonym Symbols
D1; 5DI; ITDI1; TXDI1
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NCBI Protein Information
type I iodothyronine deiodinase
UniProt Protein Name
Type I iodothyronine deiodinase
UniProt Synonym Protein Names
5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase
Protein Family
Type I iodothyronine deiodinase
UniProt Gene Name
Dio1 [Similar Products]
UniProt Synonym Gene Names
Itdi1; Txdi1 [Similar Products]
UniProt Entry Name
IOD1_MOUSE
NCBI Summary for DIO1
The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). This protein contains a selenocysteine (Sec) residue, which is necessary for maximal enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Impaired expression of this gene results in hypothyroidism, which causes severe defects in the development of many different tissues. [provided by RefSeq, Jul 2008]
UniProt Comments for DIO1
DIO1: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney. Belongs to the iodothyronine deiodinase family. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Membrane protein, integral; EC 1.97.1.10
Cellular Component: membrane; endoplasmic reticulum; integral to membrane
Molecular Function: selenium binding; thyroxine 5'-deiodinase activity; oxidoreductase activity
Biological Process: hormone biosynthetic process
Research Articles on DIO1
1. Within the supportive environment of a research vivarium, mice lacking all three deiodinases can be bred and survive.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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