ORC1L, Antibody

Origin recognition complex subunit 1; Replication control protein 1; ORC1L; ORC1; PARC1

For Research Use Only. Not for use in diagnostic procedures.

ORC1L antibody detects endogenous levels of total ORC1L protein.

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year.

Small volumes of anti-ORC1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000
ELISA: 1:5000

Western blot analysis of extracts from HepG2 cells, using ORC1L antibody.

97,350 Da

origin recognition complex subunit 1

origin recognition complex subunit 1

Origin recognition complex subunit 1

ORC1L; PARC1  [Similar Products]

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Gavin K.A., Science 270:1667-1671(1995). Wolf D.A., Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases. Gregory S.G., Nature 441:315-321(2006).

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