Product Name
IFN gamma, Monoclonal Antibody
Full Product Name
anti-human IFN gamma -R-PE
Product Synonym Names
Monoclonal anti-human IFN-gamma/R-PE
Product Gene Name
anti-IFN gamma antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2 ml (lot specific)
Performance
Ficoll prepared human peripheral blood lymphocytes were stimulated 4 hours in the presence of 50 ng/ml PMA, 2 muM ionomicin and 5 muM monensin. Cells were then harvested, washed and fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. Fixed cells were permeabolized 10 minutes in a buffer containing 0.3% Saponin. Subsequent reagent incubations and washes were done using this buffer. Five x 105 cells per tube were preincubated 5 minutes with 20 mul of 250 mug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 mul of anti-IFN-gamma/FITC at a 1:50 dilution factor (5mug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 9% sub population of the cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was 90% blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-IFNgamma antibody.
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 250 mug/ml with an A565/A280 ratio of 3.69.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Recombinant human IFN-gamma
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Other Notes
Small volumes of anti-IFN gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IFN gamma antibody
Information: The cytokine human interferon-gamma (IFN-gamma) is a glycosylated protein that exists as a homodimer of 34 kd in its
biologically active form. IFN-gamma is expressed upon activation by TH1 cells CD8+ cells and NK cells (2). Secreted IFN-gamma is a potent inhibitor of in vitro hematopoiesis and appears to serve as an important regulator of lymphocyte and macrophage function by exerting antiproliferative effects (1). Antibody ANC2E11 binds to recombinant and cellular IFN-gamma in EIA and Flow cytometry.
Applications Tested/Suitable for anti-IFN gamma antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-IFN gamma antibody
1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test)
NCBI/Uniprot data below describe general gene information for IFN gamma. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000610.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000619.2
[Other Products]
UniProt Secondary Accession #
Q53ZV4; B5BU88[Other Products]
UniProt Related Accession #
P01579[Other Products]
Molecular Weight
19,348 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG [Similar Products]
NCBI Official Synonym Symbols
IFG; IFI
[Similar Products]
NCBI Protein Information
interferon gamma; IFN-gamma; immune interferon
UniProt Protein Name
Interferon gamma
UniProt Synonym Protein Names
Immune interferon
UniProt Gene Name
IFNG [Similar Products]
UniProt Synonym Gene Names
IFN-gamma [Similar Products]
UniProt Entry Name
IFNG_HUMAN
NCBI Summary for IFN gamma
This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
UniProt Comments for IFN gamma
IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.
Protein type: Secreted; Membrane protein, integral; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; positive regulation of osteoclast differentiation; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion
Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To
Product References and Citations for anti-IFN gamma antibody
1) H.A. Young & K.J. Hardy (1995) J Leuk Biol 58:373-381. 2) A. Bilkiau (1996) Adv Immunol 62:61-130. 3) T. Sato, et al (1997) Blood 90:4749-4758.
Research Articles on IFN gamma
1. the results indicate that in tegumentary leishmaniasis caused by L. braziliensis, IFN-gamma and TNF-alpha are the main cytokines involved in the tissue damage observed in this disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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