insulin-like growth factor binding protein, acid labile subunit, ELISA Kit

Human Insulin-like growth factor-binding protein complex acid labile subunit (IGFALS) ELISA kit; ALS; insulin-like growth factor binding protein complex acid labile chain; insulin-like growth factor binding protein; acid labile subunit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human IGFALS. No significant cross-reactivity or interference between Human IGFALS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of IGFALS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS942478 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin-like growth factor binding protein, acid labile subunit (IGFALS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGFALS. The ELISA analytical biochemical technique of the MBS942478 kit is based on IGFALS antibody-IGFALS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFALS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFALS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGFALS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFALS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGFALS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFALS bound in the initial step. The color development is stopped and the intensity of the color is measured.

66,035 Da

insulin-like growth factor binding protein, acid labile subunit

insulin-like growth factor-binding protein complex acid labile subunit; insulin-like growth factor binding protein complex acid labile chain

Insulin-like growth factor-binding protein complex acid labile subunit

ALS; ALS  [Similar Products]

ALS_HUMAN

The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

IGFALS: Involved in protein-protein interactions that result in protein complexes, receptor-ligand binding or cell adhesion.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding

Biological Process: cellular protein metabolic process; signal transduction; cell adhesion

Disease: Acid-labile Subunit Deficiency

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