IFN-gamma, Monoclonal Antibody

Anti-Human IFN-gamma

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Purified

At -20 degree C

Small volumes of anti-IFN-gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Produced in BALB/c mice using highly pure recombinant human IFN-gamma as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

ELISA (EIA), Western Blot (WB)

Sandwich ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human IFN-gamma when used with biotinylated antigen affinity purified anti-human IFN-gamma as the detection antibody at a concentration of approximately 2.0-4.0 mug/ml.

Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.

19,348 Da

interferon, gamma

interferon gamma; IFN-gamma; immune interferon

Interferon gamma

IFN-gamma  [Similar Products]

IFNG_HUMAN

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Membrane protein, integral; Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of interleukin-23 production; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; sensory perception of mechanical stimulus; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To

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