Product Name
IFN-gamma, Polyclonal Antibody
Full Product Name
Biotinylated Anti-rat IFN-gamma
Product Synonym Names
Biotinylated Anti-Rat IFN-gamma
Product Gene Name
anti-IFN-gamma antibody
[Similar Products]
Matching Pairs
Capture Antibody: IFN-gamma (MBS695355)
Detection Antibody: IFN-gamma (MBS695572)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01581
Immunogen
Recombinant Rat IFN-gamma
Preparation and Storage
At -20 degree C
Other Notes
Small volumes of anti-IFN-gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IFN-gamma antibody
Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IFN-gamma. Anti-Rat IFN-gamma specific antibody was purified by affinity chromatography employing immobilized Rat IFN-gamma matrix.
Applications Tested/Suitable for anti-IFN-gamma antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-IFN-gamma antibody
Direct Detection: To detect Rat IFN-gamma by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.
Sandwich ELISA: To detect Rat IFN-gamma by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IFN-gamma as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.
Western Blot: To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
NCBI/Uniprot data below describe general gene information for IFN-gamma. It may not necessarily be applicable to this product.
NCBI Accession #
P01581.1
[Other Products]
UniProt Primary Accession #
P01581
[Other Products]
UniProt Related Accession #
P01581[Other Products]
Molecular Weight
17,918 Da
NCBI Official Full Name
Interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
Ifng [Similar Products]
NCBI Official Synonym Symbols
IFNG2
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NCBI Protein Information
interferon gamma; IFN-gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
Ifng [Similar Products]
UniProt Synonym Gene Names
IFN-gamma [Similar Products]
UniProt Entry Name
IFNG_RAT
NCBI Summary for IFN-gamma
an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]
UniProt Comments for IFN-gamma
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Research Articles on IFN-gamma
1. The findings of the present study suggest that part of ischemic injury in the embolic stroke may be mediated through the increased levels of inflammatory cytokines.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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