IFN-gamma, Polyclonal Antibody

Biotinylated Anti-Murine IFN-gamma

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Biotin

At -20 degree C

Small volumes of anti-IFN-gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IFN-gamma. Anti-Murine IFN-gamma specific antibody was purified by affinity chromatography and then biotinylated.

ELISA (EIA), Western Blot (WB)

Direct Detection: To detect Murine IFN-gamma by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IFN-gamma.

Sandwich ELISA: To detect Murine IFN-gamma by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IFN-gamma as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IFN-gamma.

Western Blot: To detect Murine IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

17,907 Da

interferon gamma

interferon gamma; IFN-gamma; gamma interferon

Interferon gamma

IFN-gamma  [Similar Products]

IFNG_MOUSE

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted, signal peptide; Secreted; Membrane protein, integral; Cytokine

Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane

Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; response to virus; defense response to protozoan; positive regulation of tumor necrosis factor production; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 biosynthetic process; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; adaptive immune response; regulation of immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of interleukin-12 biosynthetic process; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion

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