Adipocyte enhancer-binding protein 1 (AEBP1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AEBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7230803 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AEBP1. The ELISA analytical biochemical technique of the MBS7230803 kit is based on AEBP1 antibody-AEBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AEBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AEBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

82,183 Da

AE binding protein 1

adipocyte enhancer-binding protein 1; aortic carboxypeptidase-like protein

Adipocyte enhancer-binding protein 1

ACLP; AE-binding protein 1  [Similar Products]

AEBP1_HUMAN

This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma. [provided by RefSeq, May 2013]

AEBP1: May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I- kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleus

Molecular Function: calmodulin binding; carboxypeptidase activity; zinc ion binding; metallocarboxypeptidase activity; transcription corepressor activity; transcription factor activity

Biological Process: muscle development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; proteolysis; skeletal development

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