TLR2, Polyclonal Antibody

Toll-like receptor 2; CD282; Tlr2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This Mouse TLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-749 amino acids from the C-terminal region of mouse TLR2.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-Tlr2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TLR2 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.

Cardiovascular; Immunology; Microbiology; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of anti-TLR2 Pab (MBS9213149) pre-incubated without and with blocking peptide (BP#1502c) in CEM cell line lysate. TLR2-V735(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Mouse Tlr2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

89449

toll-like receptor 2

toll-like receptor 2

Toll-like receptor 2

TLR2_MOUSE

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Apoptosis; Motility/polarity/chemotaxis; Cell surface; Receptor, misc.; Membrane protein, integral

Cellular Component: cell surface; cell projection; membrane; cytoplasm; integral to membrane; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; triacylated lipoprotein binding; protein heterodimerization activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-12 production; positive regulation of leukocyte migration; microglial cell activation; positive regulation of NF-kappaB import into nucleus; leukotriene metabolic process; response to molecule of fungal origin; detection of triacylated bacterial lipoprotein; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; negative regulation of cell proliferation; response to peptidoglycan; response to molecule of bacterial origin; positive regulation of interferon-beta production; response to bacterial lipoprotein; positive regulation of oligodendrocyte differentiation; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; negative regulation of interleukin-12 production; immune system process; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of interleukin-17 production; myelin formation in the central nervous system; response to bacterium; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; chloramphenicol transport; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response; positive regulation of cytokine secretion

Okazaki, Y., et al., Nature 420(6915):563-573 (2002). Lin, Y., et al., J. Biol. Chem. 275(32):24255-24263 (2000). Matsuguchi, T., et al., Blood 95(4):1378-1385 (2000). Heine, H., et al., J. Immunol. 162(12):6971-6975 (1999). Underhill, D.M., et al., Nature 401(6755):811-815 (1999).

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