Full Product Name
PDP1 Antibody
Product Synonym Names
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; PDP 1; Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1; PDP1; PDP; PPM2C
Product Gene Name
anti-PDP1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9P0J1
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant Human [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial protein (1-154AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-PDP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PDP1 antibody
Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
Product Categories/Family for anti-PDP1 antibody
Cancer; Tags & Cell Markers; Metabolism; Signal transduction
Applications Tested/Suitable for anti-PDP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PDP1 antibody
WB: 1:500-1:5000
IHC: 1:500-1:1000
Western Blot (WB) of anti-PDP1 antibody
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate
All lanes: PDP1 antibody at 3.6ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62, 64 KDa
Observed band size: 62 KDa
Immunohistochemistry (IHC) of anti-PDP1 antibody
IHC image of MBS7108655 diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
NCBI/Uniprot data below describe general gene information for PDP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001155251.1
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NCBI GenBank Nucleotide #
NM_001161779.1
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UniProt Primary Accession #
Q9P0J1
[Other Products]
UniProt Secondary Accession #
Q5U5K1; B3KX71; J3KPU0[Other Products]
UniProt Related Accession #
Q9P0J1[Other Products]
Molecular Weight
63,695 Da
NCBI Official Full Name
pyruvate dehyrogenase phosphatase catalytic subunit 1 isoform 2
NCBI Official Synonym Full Names
pyruvate dehyrogenase phosphatase catalytic subunit 1
NCBI Official Symbol
PDP1 [Similar Products]
NCBI Official Synonym Symbols
PDH; PDP; PDPC; PPM2A; PPM2C
[Similar Products]
NCBI Protein Information
pyruvate dehyrogenase phosphatase catalytic subunit 1
UniProt Protein Name
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
UniProt Synonym Protein Names
Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1
Protein Family
PWWP domain-containing protein
UniProt Gene Name
PDP1 [Similar Products]
UniProt Synonym Gene Names
PDP; PPM2C; PDP 1; PDPC 1 [Similar Products]
NCBI Summary for PDP1
Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
UniProt Comments for PDP1
Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
Research Articles on PDP1
1. High PDH expression is associated with glucose intolerance.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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