Product Name
IFN GAMMA (IFNG), Polyclonal Antibody
Popular Item
Full Product Name
RABBIT ANTI CHICKEN INTERFERON GAMMA:Preservative Free
Product Gene Name
anti-IFNG antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49708
Specificity
This item recognizes chicken Interferon gamma (chIFN-gamma). Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFN-gamma strongly upregulates the expression of MHC class II and iNOS genes. This item, can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta, see Weining, K.C. et al. for details.
Form/Format
Preservative Free
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Immunogen
Recombinant chicken Interferon Gamma
Buffer Solution
Antiserum Preparation
Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-IFNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IFNG antibody
MBS220076 recognizes chicken Interferon gamma (chIFN-gamma). Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFN-gamma strongly upregulates the expression of MHC class II and iNOS genes. MBS220076, can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta, see Weining, K.C. et al. for details.
Applications Tested/Suitable for anti-IFNG antibody
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes for anti-IFNG antibody
Western Blot: This item detects a band of approximately 18 kDa.
NCBI/Uniprot data below describe general gene information for IFNG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_990480.1
[Other Products]
NCBI GenBank Nucleotide #
NM_205149.1
[Other Products]
UniProt Primary Accession #
P49708
[Other Products]
UniProt Secondary Accession #
Q53YS2; D3GGX1[Other Products]
UniProt Related Accession #
P49708[Other Products]
Molecular Weight
18,953 Da[Similar Products]
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG [Similar Products]
NCBI Protein Information
interferon gamma; IFN-gamma; Interferon-gamma level; interferon-gama
UniProt Protein Name
Interferon gamma
Protein Family
Interferon
UniProt Gene Name
IFNG [Similar Products]
UniProt Synonym Gene Names
IFN-gamma [Similar Products]
UniProt Entry Name
IFNG_CHICK
UniProt Comments for IFNG
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons ().
Research Articles on IFNG
1. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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