Phospho-TSC2 (S1420), Polyclonal Antibody

Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This TSC2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1420 of human TSC2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.45 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-TSC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mutations in TSC2 lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.

Cell Biology; Metabolism; Signal Transduction; Phospho Antibodies

Dot Blot (DB), ELISA (EIA), Immunofluorescence (IF)

DB~~1:500
IF~~1:10~50

Dot blot analysis of anti-TSC2-pS1420 Phospho-specific Pab (RB13391) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Confocal immunofluorescent analysis of Phospho-TSC2-S1420 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

200608

tuberous sclerosis 2

tuberin

Tuberin

TSC4  [Similar Products]

TSC2_HUMAN

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; GAPs, misc.; GAPs; Tumor suppressor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: TSC1-TSC2 complex; Golgi apparatus; cytoskeleton; growth cone; cell soma; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; caveola; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; GTPase activator activity; phosphatase binding

Biological Process: negative regulation of MAP kinase activity; phosphoinositide 3-kinase cascade; regulation of insulin receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of cell cycle; protein heterooligomerization; heart development; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; negative regulation of cell proliferation; protein localization; positive chemotaxis; cell projection organization and biogenesis; protein import into nucleus; regulation of endocytosis; protein kinase B signaling cascade; acute-phase response; cell cycle arrest; protein homooligomerization; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of protein kinase B signaling cascade; endocytosis; vesicle-mediated transport; insulin-like growth factor receptor signaling pathway; glucose import; neural tube closure; response to hypoxia; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of phosphoinositide 3-kinase cascade

Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2

Li, Y., et al., Mol. Cell. Biol. 24(18):7965-7975 (2004). Karbowniczek, M., et al., J. Biol. Chem. 279(29):29930-29937 (2004). Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004). Birchenall-Roberts, M.C., et al., J. Biol. Chem. 279(24):25605-25613 (2004). Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).

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