Cytochrome b-c1 complex subunit 8 (UQCRQ), ELISA Kit

QCR8; QP-C; QPC; OTTHUMP00000066206; OTTHUMP00000066207; low molecular mass ubiquinone-binding protein (9.5kD); ubiquinol-cytochrome c reductase; complex III subunit VII

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Bovine UQCRQ. No significant cross-reactivity or interference between Bovine UQCRQ and analogues was observed.

Store at 2-8 degree C.

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Small volumes of UQCRQ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287374 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome b-c1 complex subunit 8 (UQCRQ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UQCRQ. The ELISA analytical biochemical technique of the MBS287374 kit is based on UQCRQ antibody-UQCRQ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UQCRQ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UQCRQ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

This assay employs a two-site sandwich ELISA to quantitate UQCRQ in samples. An antibody specific for UQCRQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UQCRQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCRQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCRQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

9,720 Da

ubiquinol-cytochrome c reductase complex III subunit VII

cytochrome b-c1 complex subunit 8

Cytochrome b-c1 complex subunit 8

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.

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