Alkaline Phosphatase, Intestinal, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ALPI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS723538 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALPI. The ELISA analytical biochemical technique of the MBS723538 kit is based on ALPI antibody-ALPI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALPI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALPI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Ubiquitously expressed alkaline phosphatase has many isozymes and is a major biomarker for stem cell pluripotency. Alkaline phosphatase Intestinal ALPi is localized to mainly crypts and brush border regions and is of special interest in developmental biology and signal transduction studies. Quantitation of this critical enzyme protein in your tissue samples over related proteins, requires selective antibodies and a specific ELISA assay kit. Our ALP ELISA kits for mouse, rat and human work well in comparative IHC studies. These products are complete with primary antibody alkaline phosphatase ALP, secondary antibodies, standard curve products category, detection reagent conjugated with ALEXA 174 fluor or AMP and stop solution. ELISA kit alkaline phosphatase is fully validated with excellent intra assay variability, middle high level - 25ng ml dynamic detection range - and unexcelled assay precision. Full details on this ELISA kit product and similar cellular biochemical assays reagents are found at MyBiosource.com.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This ALPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALPI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY ALPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALPI antibody and an ALPI-HRP conjugate. The assay sample and buffer are incubated together with ALPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALPI concentration since ALPI from samples and ALPI-HRP conjugate compete for the anti-ALPI antibody . Since the number of sites is limited, as more sites are occupied by ALPI from the sample, fewer sites are left to bind ALPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALPI concentration in each sample is interpolated from this standard curve.

56,812 Da

alkaline phosphatase, intestinal

intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase

Intestinal-type alkaline phosphatase

IAP; Intestinal alkaline phosphatase  [Similar Products]

PPBI_HUMAN

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]

ALPI: intestinal alkaline phosphatase is one of four related alkaline phosphatases. The exact physiological function of alkaline phosphatases is not known. This protein is a GPI-anchored membrane bound glycosylated enzyme that is localized to fetal and ***** intestines.

Protein type: Membrane protein, GPI anchor; EC 3.1.3.1; Cofactor and Vitamin Metabolism - folate biosynthesis; Motility/polarity/chemotaxis; Phosphatase

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protease binding; alkaline phosphatase activity; zinc ion binding; magnesium ion binding

Biological Process: dephosphorylation

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