Product Name
CCNA1, siRNA
Full Product Name
CCNA1 siRNA (Mouse)
Product Synonym Names
Cyclin-A1
Product Gene Name
CCNA1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61456
Specificity
CCNA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCNA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CCNA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CCNA1 sirna
siRNA to inhibit CCNA1 expression using RNA interference
Applications Tested/Suitable for CCNA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CCNA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001292150.1
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NCBI GenBank Nucleotide #
NM_001305221.1
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UniProt Primary Accession #
Q61456
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UniProt Secondary Accession #
Q8C5U1[Other Products]
UniProt Related Accession #
Q61456[Other Products]
Molecular Weight
47,772 Da
NCBI Official Full Name
cyclin-A1
NCBI Official Synonym Full Names
cyclin A1
NCBI Official Symbol
Ccna1 [Similar Products]
NCBI Protein Information
cyclin-A1
UniProt Protein Name
Cyclin-A1
UniProt Gene Name
Ccna1 [Similar Products]
UniProt Entry Name
CCNA1_MOUSE
UniProt Comments for CCNA1
CCNA1: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Cancer Testis Antigen (CTA)
Cellular Component: microtubule cytoskeleton; cytoskeleton; cytoplasm; nucleus
Molecular Function: protein binding; protein kinase binding
Biological Process: mitosis; meiotic cell cycle; cell division; regulation of cell cycle; multicellular organismal development; regulation of G2/M transition of mitotic cell cycle; spermatogenesis; regulation of cyclin-dependent protein kinase activity; cell cycle; cell differentiation
Research Articles on CCNA1
1. With the recent data on the functions of cyclins in somatic and stem cells, we also discussed the possibilities of further studies of cyclin A1 in mouse oocytes and perhaps in the oogonial stem cells
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
