Product Name
AKT1S1, siRNA
Full Product Name
AKT1S1 siRNA (Mouse)
Product Synonym Names
PRAS; Proline-rich AKT1 substrate 1; Proline-rich AKT substrate
Product Gene Name
AKT1S1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9D1F4
Specificity
AKT1S1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AKT1S1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AKT1S1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AKT1S1 sirna
siRNA to inhibit AKT1S1 expression using RNA interference
Applications Tested/Suitable for AKT1S1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AKT1S1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001240849.1
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NCBI GenBank Nucleotide #
NM_001253920.1
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UniProt Primary Accession #
Q9D1F4
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UniProt Secondary Accession #
A2RT52[Other Products]
UniProt Related Accession #
Q9D1F4[Other Products]
Molecular Weight
27,483 Da
NCBI Official Full Name
proline-rich AKT1 substrate 1 isoform b
NCBI Official Synonym Full Names
AKT1 substrate 1 (proline-rich)
NCBI Official Symbol
Akt1s1 [Similar Products]
NCBI Official Synonym Symbols
Lobe; Lobel; PRAS40; AI227026; 1110012J22Rik
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NCBI Protein Information
proline-rich AKT1 substrate 1
UniProt Protein Name
Proline-rich AKT1 substrate 1
Protein Family
Proline-rich AKT1 substrate
UniProt Gene Name
Akt1s1 [Similar Products]
UniProt Synonym Gene Names
Proline-rich AKT substrate [Similar Products]
UniProt Entry Name
AKTS1_MOUSE
UniProt Comments for AKT1S1
PRAS40: plays a role in phosphatidylinositol 3-kinase (PI3K)/AKT survival signaling. PRAS40 is a physiological target of in vivo insulin action. Hyperinsulinemia increased its phosphorylation in human skeletal muscle biopsies. Phosphorylated PRAS40 is predominantly localized to the nucleus. In rats fed a high-fat diet (HFD), phosphorylation of PRAS40 was markedly reduced when compared with low-fat diet-fed animals in all tissues examined. A novel mTOR binding partner that mediates Akt signals to mTOR. Binding of PRAS40 inhibits mTOR activity and suppresses constitutive activation of mTOR in cells lacking TSC2. Phosphorylation by Akt and perhaps related kinases leads to its binding to 14-3-3. Two alternatively spliced isoforms have been described.
Protein type: Apoptosis
Cellular Component: protein complex; cytoplasm; nucleus; cytosol
Biological Process: Notch signaling pathway; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of cell size
Research Articles on AKT1S1
1. PRAS40 appears to reduce brain injury following ischemic stroke by converting cell signaling from Akt to mTOR
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
