BRK1, Polyclonal Antibody

MDS027; hHBrk1; C3orf10; HSPC300

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total BRK1 protein.

Antigen affinity purification.

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

2.6 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-BRK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Gel: 10%SDS-PAGE Lysate: 40ug A172 cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/40.

12,046 Da

BRICK1, SCAR/WAVE actin-nucleating complex subunit

protein BRICK1

Protein BRICK1

C3orf10; BRK1  [Similar Products]

BRK1_HUMAN

BRK1: Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. Belongs to the BRK1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoskeleton; lamellipodium; cytosol

Molecular Function: protein complex binding; Rac GTPase binding

Biological Process: in utero embryonic development; positive regulation of cell proliferation; cell motility involved in cell locomotion; Rac protein signal transduction; innate immune response; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis

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