CD171, Monoclonal Antibody

CAML1; HSAS1; Hyd; L1 Cell Adhesion Molecule; L1-NCAM; MASA; MIC5; NCAM-L1; Nerve-growth factor-inducible large external glycoprotein; Neural cell adhesion molecule L1; NILE; S10; SPG1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1, kappa

UJ127

Mouse

Recognizes a cell surface protein of 220-240kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or ***** brain.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Store at 2 to 8 degree C. Antibody is stable for 24 months.

Small volumes of anti-L1CAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Immunohistology (IH)

ELISA (For coating, order antibody without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunoprecipitation (1-2ug/500ug protein); Western Blotting (0.5-1.0ug/ml); Immunohistology (Frozen) (1-2ug/ml for 30 min at RT Optimal dilution for a specific application should be determined.

220-240kDa

L1 cell adhesion molecule

neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1

Neural cell adhesion molecule L1

CAML1; MIC5; N-CAM-L1; NCAM-L1  [Similar Products]

L1CAM_HUMAN

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: presynaptic membrane; cell surface; focal adhesion; plasma membrane; integral to membrane; terminal button; external side of plasma membrane

Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding

Biological Process: nervous system development; axon guidance; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; blood coagulation; cell adhesion; homophilic cell adhesion; leukocyte migration

Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome

1. Kemshead J, et. al. International J Cancer, 1983; 31:187-195.
2. Patel, et. al. Biochem Soc Transactions, 1990; 18:274

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