IMPDH1, Polyclonal Antibody

Inosine-5'-monophosphate dehydrogenase 1 {ECO:0000255|HAMAP-Rule:MF_03156}; IMP dehydrogenase 1 {ECO:0000255|HAMAP-Rule:MF_03156}; IMPD 1 {ECO:0000255|HAMAP-Rule:MF_03156}; IMPDH 1 {ECO:0000255|HAMAP-Rule:MF_03156}; 111205 {ECO:0000255|HAMAP-Rule:MF_03156}; IMPDH-I; IMPDH1 {ECO:0000255|HAMAP-Rule:MF_03156}; IMPD1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This IMPDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-514 amino acids from the C-terminal region of human IMPDH1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.43 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-IMPDH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene.

Metabolism; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)

WB~~1:1000

IMPDH1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the IMPDH1 antibody detected the IMPDH1 protein (arrow).

Confocal immunofluorescent analysis of IMPDH1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

IMPDH1 Antibody (C-term) (MBS9207425)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IMPDH1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

55406

IMP (inosine 5'-monophosphate) dehydrogenase 1

inosine-5'-monophosphate dehydrogenase 1

Inosine-5'-monophosphate dehydrogenase 1

IMP dehydrogenase 1; IMPD 1; IMPDH 1  [Similar Products]

IMDH1_HUMAN

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

IMPDH1: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. Defects in IMPDH1 are the cause of Leber congenital amaurosis type 11 (LCA11). LCA11 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the IMPDH/GMPR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.205; Nucleotide Metabolism - purine; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 7q31.3-q32

Cellular Component: nucleoplasm; cytoplasm; cell junction; nucleus; cytosol

Molecular Function: nucleic acid binding; DNA binding; RNA binding; metal ion binding; nucleotide binding; IMP dehydrogenase activity

Biological Process: purine ribonucleoside monophosphate biosynthetic process; lymphocyte proliferation; nucleobase, nucleoside and nucleotide metabolic process; GMP biosynthetic process; purine base metabolic process

Disease: Leber Congenital Amaurosis 11; Retinitis Pigmentosa 10

Ohmann, E.L., et al. Pediatr Transplant 14(7):891-895(2010) Gensburger, O., et al. Pharmacogenet. Genomics 20(9):537-543(2010) Kagaya, H., et al. Basic Clin. Pharmacol. Toxicol. 107(2):631-636(2010) Ohmann, E.L., et al. J. Heart Lung Transplant. 29(5):509-516(2010) Shumei, L., et al. Adv. Exp. Med. Biol. 664, 293-297 (2010) :

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