CCND1, Polyclonal Antibody

CCND1; BCL1; D11S287E; PRAD1; U21B31

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous level of total CCND1 protein.

Antibodies were purified by affinity purification using immunogen.

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-CCND1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).Of the three D cyclins, aberrant expression of cyclin D1 has been associated with many forms of cancer including B cell lymphomas and can directly contribute to oncogenesis by various mechanisms including gene translocation or amplification (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5).

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100

Western blot analysis of extracts of HeLa cell line, using CCND1 antibody.

Immunohistochemical analysis of paraffin-embedded human epididymis using CCND1 antibody at dilution of 1:500 (400x lens).

33,729 Da

cyclin D1

G1/S-specific cyclin-D1

G1/S-specific cyclin-D1

BCL1; PRAD1; BCL-1  [Similar Products]

CCND1_HUMAN

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Nuclear receptor co-regulator; Activator; Cell cycle regulation; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; membrane; transcriptional repressor complex; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; protein kinase binding; transcription factor binding; transcription corepressor activity; protein kinase activity

Biological Process: lactation; G1 DNA damage checkpoint; fat cell differentiation; establishment and/or maintenance of chromatin architecture; negative regulation of Wnt receptor signaling pathway; re-entry into mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to vitamin E; response to magnesium ion; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; organ regeneration; Notch signaling pathway; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; liver development; response to organic nitrogen; response to ethanol; response to estrogen stimulus; cell division; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple

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