PCSK5, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Small volumes of anti-PCSK5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC)

Western blot analysis of extracts of various cell lines, using PCSK5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

913

proprotein convertase subtilisin/kexin type 5

proprotein convertase subtilisin/kexin type 5; subtilase; protease PC6; prohormone convertase 5; proprotein convertase 6; subtilisin/kexin-like protease PC5

Proprotein convertase subtilisin/kexin type 5

PC5; PC6; PC5; PC6; hPC6  [Similar Products]

PCSK5_HUMAN

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER. It then sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. This encoded protein is widely expressed and one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It mediates posttranslational endoproteolytic processing for several integrin alpha subunits and is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Alternative splicing results in multiple transcript variants, some of which encode distinct isoforms, including a protease packaged into dense core granules (PC5A) and a type 1 membrane bound protease (PC5B). [provided by RefSeq, May 2014]

PCSK5: Likely to represent a widespread endoprotease activity within the constitutive and regulated secretory pathway. Capable of cleavage at the RX(K/R)R consensus motif. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2 and CALD1. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 9q21.3

Cellular Component: Golgi apparatus; extracellular space; Golgi lumen; integral to membrane; secretory granule

Molecular Function: peptidase activity; serine-type endopeptidase activity; peptide binding

Biological Process: nerve growth factor receptor signaling pathway; signal peptide processing; respiratory tube development; heart development; embryonic skeletal development; cytokine biosynthetic process; peptide hormone processing; viral infectious cycle; proteolysis; limb morphogenesis; peptide biosynthetic process; anterior/posterior pattern formation; nerve growth factor processing; renin secretion into blood stream; cell-cell signaling; protein processing; kidney development; embryonic gut development; embryo implantation

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