Product Name
DDIT3, siRNA
Full Product Name
DDIT3 siRNA (Mouse)
Product Synonym Names
CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA-damage-inducible protein GADD153
Product Gene Name
DDIT3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35639
Specificity
DDIT3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DDIT3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DDIT3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DDIT3 sirna
siRNA to inhibit DDIT3 expression using RNA interference
Applications Tested/Suitable for DDIT3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DDIT3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001277112.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001290183.1
[Other Products]
UniProt Primary Accession #
P35639
[Other Products]
UniProt Secondary Accession #
Q91YW9[Other Products]
UniProt Related Accession #
P35639[Other Products]
Molecular Weight
19,189 Da
NCBI Official Full Name
DNA damage-inducible transcript 3 protein
NCBI Official Synonym Full Names
DNA-damage inducible transcript 3
NCBI Official Symbol
Ddit3 [Similar Products]
NCBI Official Synonym Symbols
chop; CHOP10; CHOP-10; gadd153
[Similar Products]
NCBI Protein Information
DNA damage-inducible transcript 3 protein
UniProt Protein Name
DNA damage-inducible transcript 3 protein
UniProt Synonym Protein Names
C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA-damage-inducible protein GADD153
Protein Family
DNA damage-inducible transcript 3 protein
UniProt Gene Name
Ddit3 [Similar Products]
UniProt Synonym Gene Names
Chop; Chop10; Gadd153; DDIT-3; CHOP; CHOP-10 [Similar Products]
UniProt Entry Name
DDIT3_MOUSE
UniProt Comments for DDIT3
CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.
Protein type: DNA-binding; Autophagy; Transcription factor; Oncoprotein
Cellular Component: nucleoplasm; cell; late endosome; cytoplasm; intracellular; cytosol; nucleus
Molecular Function: protein binding; leucine zipper domain binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription factor activity; negative regulation of fat cell differentiation; cell cycle; mRNA transcription from RNA polymerase II promoter; blood vessel maturation; response to unfolded protein; negative regulation of DNA binding; response to starvation; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation
Research Articles on DDIT3
1. Data show that vascular smooth muscle cells (VSMCs)-C/EBP-homologous protein (CHOP) deficiency decreases the proliferation of aortic explant-dderived VSMCs.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
